Services and Charges
Services
Lund Stem Cell Center members have full access to the following services and methods.
Services
- Advice on experimental design
- Sample preparation for MS: from FACS-sorted primary cells, bulk cells, cultured cells, plasma, extracellular fluid …
- LC- and MS maintenance and quality control
- MS data acquisition using Dionex Ultimate 3000 coupled to Orbitrap Exploris 480 with FAIMSPro
- MS data analysis, post-processing, data evaluation and statistical analysis
- Assistance with data interpretation
- Assistance with data visualization and presentation
- Submission of MS data to ProteomeXchange or MassIVE
Type of Proteomics Methods and Approaches
- Quantitative proteomics
- Data-dependent acquisition, Tandem Mass Tag (TMT)-labelled profiling
- Data-independent acquisition (DIA), label-free
- Analysis of post-translational modifications: phosphorylation, cysteine oxidation, ubiquitinylation
- Protein-protein interaction analysis (IP-MS)
- Immunopeptidomics
- High-pH reverse phase fractionation
- Development of new methods time-permitting*
*Targeted proteomic assay with SureQuant technology (Thermo Scientific) is underway.
Charges
| Services | Charges (SEK)* | |
|---|---|---|
| Sample preparation | DIA, label free, up to 15 samples | 4 000 |
| TMT 6 plex | 7 000 | |
| TMT 11-plex | 8 400 | |
| TMTPro (16-plex) | 15 400 | |
| Phosphopeptide-enrichment, up to 15 samples | 4 000 | |
| High-pH reverse phase fractionation | 1 000 | |
| MS running costs | First day of MS analysis (fixed) | 6 000 |
| hourly rate after 24th hour | 250 | |
| Statistics, data analysis and visualization | Per project, up to 8 hours work time | 5 000 |
| Per project, > 8 hours work time | 8 000 | |
| Consultation | free | |
*MS calibration, QC runs and cleaning between studies are not charged. Cleaning within studies are charged.
Book Our Services:
For bookings and consultations, please contact:
Jenny [dot] Hansson [at] med [dot] lu [dot] se (Jenny[dot]Hansson[at]med[dot]lu[dot]se)
