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Course: Gene-editing technologies

Are you new to working with gene-editing? We will teach you how! We offer a new course containing in depth information about gene editing design, execution and delivery.

DNA i blått

The aim of this 5-day course is to get a better theoretical and practical understanding of gene-editing. From experimental design to edit evaluation and post-edit quality controls. 

Target group: Anyone (from TAs and PhDs to PIs) who is new to working with gene-editing. 

Please note that this  course is not yet available to for inclusion in LAKOK, so no credits, only a certificate, will be awarded. Please note that even though persons from all universities are welcome to apply, local applicants are prioritised. 

Course Content

Focus lectures

  • How to design SNP and small edits, KOs, larger insertions and deletions
  • How to peform the edit - components, delivery, (eg. RNP vs plasmid, electroporation vs lipofection vs virus)
  • How to evaluate the edit in and clonal situations
  • What´s next? Edit type specific quality controls and other QC
  • The workflow and reporting
  • Experimental design, numbers, read-out etc

Overview lectures

  • History, background and editing alternatives
  • The crispr toolbox overview
  • CRISPRa/i
  • Prime and base-editing
  • CRISPR screens

Practical elements

  • Design
  • Analyze bulk-edited cells
  • Analyze individual clones

Instructors

Dr. Pia Johansson, Cell and Gene Therapy Core Facility

+ guest lecturers

Course Fee 

(for national academic users, external customers please contact us for availability and pricing)

  • Full course: 7 500 SEK

 

 

Next course

Date: May 12-16, 2025

Venue: Lund Stem Cell Center, Lund University. 

Registration

Registration opens: February 04th, 2025

Registration closes: March 31st, 2025 (or when full)

The course is open to all applicants but priority will be given to Lund Stem Cell Center and Lund University applicants. Applicants will be informed of the decision latest one week after the closure of registration.

4 Feb 2025